antidermatophytic and toxicological evaluations of dichloromethane-methanol extract, fractions and compounds isolated from coula edulis

نویسندگان

jean de dieu tamokou departments of biochemistry, faculty of sciences, university of dschang, dschang, cameroon

jules roger kuiate departments of biochemistry, faculty of sciences, university of dschang, dschang, cameroon

donatien gatsing departments of biochemistry, faculty of sciences, university of dschang, dschang, cameroon

alango pépin nkeng efouet chemistry, faculty of sciences, university of dschang, dschang, cameroon

چکیده

background: coula edulis bail (olacaceae), is an evergreen tree growing to a height of 25-38 m. this study aimed at evaluating the antidermatophytic and toxicological properties of the stem bark of c. edulis extract as well as fractions and compounds isolated from it. methods: the plant extract was prepared by maceration in ch2cl2-meoh (1:1 v/v). the fractionation of this extract was done by silica gel column chromatography. antidermatophytic activities were assayed using agar dilution method. the acute and sub-acute toxicities of oral administrations of the extract were studied in rodents. results: the crude extract of c. edulis displayed antidermatophytic activity against the tested microorganisms with highest activity against microsporum audouinii and trichophyton mentagrophytes. the fractionation enhanced the antidermatophytic activity in fraction f3 (mic=0.62-1.25 mg/ml) compared to the crude extract (mic=1.25-5 mg/ml). further fractiona-tion and purification of the fractions f2 and f3 gave respectively 3-o-β-d-glucopyranoside of sitosterol (mic=0.20-0.40 mg/ml) and a mixture of β-sitosterol, stigmasterol and n-hexadecanoid acid (mic=0.80 mg/ml). the median lethal doses (ld50) of the crude extract were 16.8 and 19.6 g/kg body weight (bw) in male and female mice, respectively. at 200 mg/kg bw, there was a decrease in body weight gain, food and water consumptions. gross anatomical analysis revealed white vesicles on the liver of the rats treated with the extract at 200 mg/kg bw. this dose also induced significant (p = 200 mg/kg bw), the extract has significant hepatotoxic and nephrotoxic activities.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Antidermatophytic and Toxicological Evaluations of Dichloromethane-Methanol Extract, Fractions and Compounds Isolated from Coula edulis

Background: Coula edulis Bail (Olacaceae), is an evergreen tree growing to a height of 25-38 m. This study aimed at evaluating the antidermatophytic and toxicological properties of the stem bark of C. edulis extract as well as fractions and compounds isolated from it. Methods: The plant extract was prepared by maceration in CH2Cl2-MeOH (1:1 v/v). The fractionation of this extract was do...

متن کامل

Antidermatophytic and Toxicological Evaluations of Dichloromethane-Methanol Extract, Fractions and Compounds Isolated from Coula edulis

BACKGROUND Coula edulis Bail (Olacaceae), is an evergreen tree growing to a height of 25. This study aimed at evaluating the antidermatophytic and toxicological properties of the stem bark of C. edulis extract as well as fractions and compounds isolated from it. METHODS The plant extract was prepared by maceration in CH(2)Cl(2)-MeOH (1:1 v/v). The fractionation of this extract was done by sil...

متن کامل

extraction and characterization of allium irancum plant extract and its application in the green synthesis of silver nano particles and oxidation of thiocarbony1 compounds

سنتز سبز نانوذرات فلزی (nps) درسالهای اخیر توجه بسیارزیادی را به خود جلب کرده است. زیرا این پروتوکل کم هزینه وسازگار با محیط زیست از روش های استاندارد سنتز. در این پایان نامه ما گزارش میکنیم یک روش ساده و سازگار با محیط زیست برای سنتز نانوذرات نقره با استفاده از محلول آبی عصاره گیاه allium iranicum به عنوان یک عامل کاهش دهنده ی طبیعی. نانو ذرات نقره مشخص شد با استفاده از تکنیک های uv-visible، x...

Antibacterial activities of the methanol extract, fractions and compounds from Elaeophorbia drupifera (Thonn.) Stapf. (Euphorbiaceae)

BACKGROUND Elaeophorbia drupifera (Thonn.) Stapf. (Euphorbiaceae) is used in Cameroonian folk medicine to treat several ailments including bacterial-related diseases such as skin infections. In this study, the methanol extract from the leaves (EDL), fractions (EDLa-d), sub-fractions EDLc1-7 and EDLc31-35 as well as isolated compounds were tested for their antimicrobial activities against a pane...

متن کامل

Antimicrobial activities of the methanol extract, fractions and compounds from Ficus polita Vahl. (Moraceae)

BACKGROUND Many plants of the family Moraceae are used in the treatment of infectious diseases. Ficus polita Vahl., an edible plant belonging to this family is used traditionally in case of dyspepsia, infectious diseases, abdominal pains and diarrhea. The present work was designed to assess the antimicrobial activity of the methanol extract from the roots of F. polita (FPR), as well as that of ...

متن کامل

Isolation and identification of compounds from toxic fractions of Cuminum cyminum extract

Background and objectives: Cuminum cyminum from Apiaceae family is a common medicinal plant. The fruits of this plant are the second most popular spice after Piper nigrum in the world. It is used in cooking as flavoring and also it has been used for treatment of toothache, epilepsy, diarrhea,emphysema and gastrointestinal problems in Iranian traditional medici...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of medical sciences

جلد ۳۶، شماره ۲، صفحات ۱۱۱-۰

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023